Regulatory

Part:BBa_J70592:Design

Designed by: Joseph Lynch   Group: Knight Lab   (2010-06-18)

RFC12 Constitutive Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There was a NheI site present at bp 7, so a silent mutation was introduced at that location to change a T to a G.

Designed with synthetic oligos: 

5' AATTC GCGGCCGC T ACTAGT TTGACGGCGAGCTCAGTCCTAGGTACAGTGCTAGC GCTAGC A GCGGCCG CTGCA 3', Prom-F
5' GCGGCCGCTGCTAGC GCTAGCACTGTACCTAGGACTGAGCTCGCCGTCAA ACTAGTAGCGGCCGCG 3', Prom-R

Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.

Source

BBa_J23100

References